1D6M
CRYSTAL STRUCTURE OF E. COLI DNA TOPOISOMERASE III
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003916 | molecular_function | DNA topoisomerase activity |
| A | 0003917 | molecular_function | DNA topoisomerase type I (single strand cut, ATP-independent) activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0006261 | biological_process | DNA-templated DNA replication |
| A | 0006265 | biological_process | DNA topological change |
| A | 0006281 | biological_process | DNA repair |
| A | 0006310 | biological_process | DNA recombination |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0043597 | cellular_component | cytoplasmic replication fork |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051304 | biological_process | chromosome separation |
| A | 0098847 | molecular_function | sequence-specific single stranded DNA binding |
Functional Information from PROSITE/UniProt
| site_id | PS00396 |
| Number of Residues | 16 |
| Details | TOPO_IA_1 Topoisomerase (Topo) IA-type active site signature. QkLYEthkl........ITYpRSD |
| Chain | Residue | Details |
| A | GLN317-ASP332 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 133 |
| Details | Domain: {"description":"Toprim","evidences":[{"source":"HAMAP-Rule","id":"MF_00953","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | Region: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01383","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10574789","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00953","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Site: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 64 |
| Chain | Residue | Details |
| A | GLU7 | hydrogen bond acceptor, hydrogen bond donor, metal ligand, proton acceptor, proton donor |
| A | LYS8 | electrostatic stabiliser, hydrogen bond donor |
| A | ASP103 | metal ligand |
| A | ASP105 | metal ligand |
| A | TYR328 | hydrogen bond acceptor, nucleofuge, nucleophile |
| A | ARG330 | electrostatic stabiliser, hydrogen bond donor |
