1D6M
CRYSTAL STRUCTURE OF E. COLI DNA TOPOISOMERASE III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1995-03-15 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 236.000, 236.000, 108.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.26800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.260 * |
| Total number of observations | 136735 * | |
| Number of reflections | 33563 * | |
| <I/σ(I)> | 0.072 | |
| Completeness [%] | 95.9 | 85.5 |
| Redundancy | 4.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.3 | 14 * | 1.65M Na, potassium phosphate, pH 6.3, MICRODIALYSIS, temperature 287K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Na,K phosphate | 1.65 (M) |
