1CZA
MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP
Functional Information from GO Data
Chain | GOid | namespace | contents |
N | 0001678 | biological_process | intracellular glucose homeostasis |
N | 0002720 | biological_process | positive regulation of cytokine production involved in immune response |
N | 0004340 | molecular_function | glucokinase activity |
N | 0004396 | molecular_function | hexokinase activity |
N | 0005515 | molecular_function | protein binding |
N | 0005524 | molecular_function | ATP binding |
N | 0005536 | molecular_function | D-glucose binding |
N | 0005737 | cellular_component | cytoplasm |
N | 0005739 | cellular_component | mitochondrion |
N | 0005741 | cellular_component | mitochondrial outer membrane |
N | 0005829 | cellular_component | cytosol |
N | 0005975 | biological_process | carbohydrate metabolic process |
N | 0006002 | biological_process | fructose 6-phosphate metabolic process |
N | 0006006 | biological_process | glucose metabolic process |
N | 0006013 | biological_process | mannose metabolic process |
N | 0006096 | biological_process | glycolytic process |
N | 0006954 | biological_process | inflammatory response |
N | 0008865 | molecular_function | fructokinase activity |
N | 0009298 | biological_process | GDP-mannose biosynthetic process |
N | 0016301 | molecular_function | kinase activity |
N | 0016310 | biological_process | phosphorylation |
N | 0016773 | molecular_function | phosphotransferase activity, alcohol group as acceptor |
N | 0019158 | molecular_function | mannokinase activity |
N | 0019318 | biological_process | hexose metabolic process |
N | 0032731 | biological_process | positive regulation of interleukin-1 beta production |
N | 0042834 | molecular_function | peptidoglycan binding |
N | 0045087 | biological_process | innate immune response |
N | 0045121 | cellular_component | membrane raft |
N | 0046835 | biological_process | carbohydrate phosphorylation |
N | 0047931 | molecular_function | glucosamine kinase activity |
N | 0051156 | biological_process | glucose 6-phosphate metabolic process |
N | 0061621 | biological_process | canonical glycolysis |
N | 0061728 | biological_process | GDP-mannose biosynthetic process from mannose |
N | 0072655 | biological_process | establishment of protein localization to mitochondrion |
N | 0072656 | biological_process | maintenance of protein location in mitochondrion |
N | 0141199 | biological_process | GDP-mannose biosynthetic process from glucose |
N | 1901135 | biological_process | carbohydrate derivative metabolic process |
Functional Information from PROSITE/UniProt
site_id | PS00378 |
Number of Residues | 26 |
Details | HEXOKINASE_1 Hexokinase domain signature. VGFTFSFPcqqskIDeaiLitWTKrF |
Chain | Residue | Details |
N | VAL150-PHE175 | |
N | LEU598-PHE623 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1DGK, ECO:0007744|PDB:1QHA |
Chain | Residue | Details |
N | ARG30 | |
N | ARG425 |
site_id | SWS_FT_FI2 |
Number of Residues | 2 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9735292, ECO:0007744|PDB:1DGK, ECO:0007744|PDB:1HKC |
Chain | Residue | Details |
N | ASP84 | |
N | THR863 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0000269|PubMed:9735292, ECO:0007744|PDB:1DGK, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1HKC, ECO:0007744|PDB:4F9O, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA |
Chain | Residue | Details |
N | SER155 |
site_id | SWS_FT_FI4 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0000269|PubMed:9735292, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1DGK, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1HKC, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4F9O, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA, ECO:0007744|PDB:4FPB |
Chain | Residue | Details |
N | THR172 | |
N | ASN208 | |
N | ASN235 | |
N | GLU260 | |
N | GLN291 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA |
Chain | Residue | Details |
N | ASP209 |
site_id | SWS_FT_FI6 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4F9O, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA, ECO:0007744|PDB:4FPB |
Chain | Residue | Details |
N | THR232 | |
N | ASP413 | |
N | SER449 | |
N | THR680 | |
N | ASP861 |
site_id | SWS_FT_FI7 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0007744|PDB:1QHA |
Chain | Residue | Details |
N | ASN345 |
site_id | SWS_FT_FI8 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA |
Chain | Residue | Details |
N | ASP532 |
site_id | SWS_FT_FI9 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0007744|PDB:1DGK, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:4F9O, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA, ECO:0007744|PDB:4FPB |
Chain | Residue | Details |
N | SER603 |
site_id | SWS_FT_FI10 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1DGK, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4F9O, ECO:0007744|PDB:4FOE, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA, ECO:0007744|PDB:4FPB |
Chain | Residue | Details |
N | THR620 | |
N | ASN656 | |
N | SER682 | |
N | GLU708 | |
N | GLU742 |
site_id | SWS_FT_FI11 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA |
Chain | Residue | Details |
N | ASP657 |
site_id | SWS_FT_FI12 |
Number of Residues | 2 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10686099, ECO:0007744|PDB:1DGK |
Chain | Residue | Details |
N | GLY747 | |
N | THR784 |
site_id | SWS_FT_FI13 |
Number of Residues | 1 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10574795, ECO:0000269|PubMed:10686099, ECO:0000269|PubMed:9493266, ECO:0007744|PDB:1CZA, ECO:0007744|PDB:1HKB, ECO:0007744|PDB:1QHA, ECO:0007744|PDB:4F9O, ECO:0007744|PDB:4FOI, ECO:0007744|PDB:4FPA, ECO:0007744|PDB:4FPB |
Chain | Residue | Details |
N | SER897 |
site_id | SWS_FT_FI14 |
Number of Residues | 1 |
Details | MOD_RES: N-acetylmethionine => ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:25944712 |
Chain | Residue | Details |
N | MET1 |
site_id | SWS_FT_FI15 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P05708 |
Chain | Residue | Details |
N | SER337 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 3 |
Details | M-CSA 696 |
Chain | Residue | Details |
N | ARG539 | electrostatic stabiliser, polar interaction |
N | SER603 | electrostatic stabiliser, polar interaction |
N | ASP657 | proton acceptor, proton donor |