1CZA
MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-09-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 145.660, 145.800, 58.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 * |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.26800 * |
RMSD bond length | 0.016 * |
RMSD bond angle | 0.036 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.048 * | 0.410 * |
Total number of observations | 286697 * | |
Number of reflections | 83349 * | |
<I/σ(I)> | 16.8 | |
Completeness [%] | 98.6 * | 82.3 * |
Redundancy | 4.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 * | 298 | PEG 8000, SODIUM ACETATE, MES, ADP, ALUMINUM NITRATE, SODIUM FLUORIDE, MAGNESIUM ACETATE, GLUCOSE, pH 6.5, EVAPORATION, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 20 (mg/ml) | |
2 | 1 | 2 | PEG8000 | 14-18 (%) | |
3 | 1 | 2 | MES | 100 (mM) | |
4 | 1 | 2 | sodium acetate | 150-250 (mM) | |
5 | 1 | 2 | ADP | 20 (mM) |