9ZB7

A DARPin fused to the 1TEL crystallization chaperone via a direct helical fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 65
Unit cell lengths	83.690, 83.690, 58.491
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.830 - 1.240
R-factor	0.1467
Rwork	0.146
R-free	0.17020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.830	1.284
High resolution limit [Å]	1.240	1.240
Rmerge	0.081	2.081
Rmeas	0.087	2.246
Rpim	0.033	0.837
Number of reflections	65882	6504
<I/σ(I)>	9.46	1.41
Completeness [%]	99.8	99.15
Redundancy	6.9	6.9
CC(1/2)	0.997	0.261

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	298	0.4 M Ammonium phosphate monobasic