9TZ5
Triple Stack Doxycycline Bound 50S Subunit of the Coxiella burnetii Ribosome
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.22 |
| Software | crYOLO,Coot,REFMAC,cryoSPARC,EPU,UCSF ChimeraX,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
