9QU1

Crystal structure of the human RalGAPA2 N-terminal domain with human kappaB-Ras1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-28
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.68879
Spacegroup name	C 1 2 1
Unit cell lengths	163.564, 63.430, 104.163
Unit cell angles	90.00, 91.26, 90.00

Refinement procedure

Resolution	63.630 - 2.720
R-factor	0.1976
Rwork	0.195
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.529
Data reduction software	XDS (BUILT=20220820)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.138	2.959
High resolution limit [Å]	2.716	2.716
Rmerge	0.114	1.202
Rmeas	0.125	1.363
Rpim	0.052	0.632
Number of reflections	21842	1093
<I/σ(I)>	10.2
Completeness [%]	91.5	57.9
Redundancy	5.56	4.56
CC(1/2)	0.997	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	17 % PEG 3350 20 % glycerol 0.1 M MgCl2 1 ul protei + 1 ul screening buffer + 1 ul seed seed (1:200)