9QK2
Structure of the Complement classical and lectin pathway C3 convertase in complex with substrate C3
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS TALOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Software | cryoSPARC,PHENIX,EPU,Coot,UCSF ChimeraX |
| Symmetry | C1 |
| CTF correction | NONE |
