9QCD

Micro-ED structure of the NSH2-CSH2 tandem domain of SHP2 in complex with the bis-phosphorylated pY627-pY659-Gab1 (613-694) peptide

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	30.595, 82.253, 117.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.720 - 3.200
R-factor	0.3032
Rwork	0.300
R-free	0.35270
RMSD bond length	0.003
RMSD bond angle	0.648
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	4.030
High resolution limit [Å]	3.200	3.200
Rmeas	0.802	2.200
Total number of observations	56190
Number of reflections	4729	744
<I/σ(I)>	3.29	1.41
Completeness [%]	88.8
Redundancy	11.9	12.3
CC(1/2)	0.940	0.530