9OU8

Crystal structure of hUGDH Dimer-A104L/A136M complexed with UDP-Xyl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-03
Detector	MAR CCD 300 mm
Wavelength(s)	1.000
Spacegroup name	H 3
Unit cell lengths	179.630, 179.630, 186.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.080 - 2.410
R-factor	0.1816
Rwork	0.180
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.221
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.100	2.440
High resolution limit [Å]	2.410	2.410
Number of reflections	86641	6352
<I/σ(I)>	11.7	1.5
Completeness [%]	100.0	99.99
Redundancy	8.6	8.7
CC(1/2)	0.997	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	0.9 M Ammonium sulfate, 0.75 M Lithium sulfate, 0.1 M Sodium citrate pH 6.2