9OC7

Crystal structure of FcRn in complex with Human Astrovirus 2 spike

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	174.878, 86.217, 157.087
Unit cell angles	90.00, 122.08, 90.00

Refinement procedure

Resolution	29.520 - 3.070
R-factor	0.1963
Rwork	0.194
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	FAST_DP
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.520	3.150
High resolution limit [Å]	3.070	3.070
Number of reflections	37066	2718
<I/σ(I)>	11.5
Completeness [%]	99.6
Redundancy	7.1
CC(1/2)	0.990	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.9	293	25% 1,2-propanediol, 10% glycerol, 5% PEG-3000, 0.1 M phosphate-citrate