9N1Z

Structure of C3d Bound to a Fragment of FHR-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	113.302, 116.228, 165.582
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.570 - 2.310
R-factor	0.1957
Rwork	0.194
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.265	0.084	0.951
Rmeas	0.277	0.088	1.021
Rpim	0.079	0.025	0.356
Total number of observations	537249
Number of reflections	47909	5052	4614
<I/σ(I)>	6.7
Completeness [%]	99.2	99.8	96.9
Redundancy	11.2	12	7.6
CC(1/2)	0.946	0.995	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.075 M HEPES [PH 7.5] 1.2 M Ammonium Sulfate