9LP8
Extrinsic-protein reconstituted PSII of Thermosynechococcus vulcanus NIES-2134
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.342, 228.882, 286.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.100 - 2.000 |
| R-factor | 0.1535 |
| Rwork | 0.152 |
| R-free | 0.17780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wu2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.970 |
| Rmeas | 0.053 | 0.970 |
| Rpim | 0.019 | 0.344 |
| Number of reflections | 542391 | 53767 |
| <I/σ(I)> | 23.52 | |
| Completeness [%] | 100.0 | 99.96 |
| Redundancy | 7.7 | 7.8 |
| CC(1/2) | 1.000 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 5% PEG1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES buffer, pH 6.0, MICRO-BATCH METHOD UNDER OIL |
