9L88

Structure of human TREX1-DNA complex with K66R disease-associated mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	44.257, 92.712, 160.551
Unit cell angles	90.00, 90.89, 90.00

Refinement procedure

Resolution	32.220 - 2.620
R-factor	0.1965
Rwork	0.195
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tqq
RMSD bond length	0.004
RMSD bond angle	0.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.220	2.690
High resolution limit [Å]	2.620	2.620
Rpim	0.070	0.322
Number of reflections	38765	3673
<I/σ(I)>	11.3	2.1
Completeness [%]	99.3	93.33
Redundancy	8.3	7.5
CC(1/2)	0.976	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.05 M Li2SO4, 22.5% PEG-3350, 0.1M Bis-Tris pH 6.5