Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2023-12-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.610, 71.610, 153.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.450 - 1.400 |
| R-factor | 0.22899 |
| Rwork | 0.228 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.969 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.880 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.044 | 0.196 |
| Number of reflections | 79055 | 3849 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 25mM HEPES pH7.5, 1.8-2.0 M LiSO4, 10 Mm CoCl2 |
