9K0I

Crystal structure of FGFR4 kinase domain with 32a

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2022-04-21
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 1 21 1
Unit cell lengths	42.174, 61.875, 59.868
Unit cell angles	90.00, 97.83, 90.00

Refinement procedure

Resolution	42.817 - 2.236
R-factor	0.1785
Rwork	0.176
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.801
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.820	2.316
High resolution limit [Å]	2.236	2.236
Rmerge	0.201	0.324
Rmeas	0.224
Number of reflections	14865	1447
<I/σ(I)>	7.79	4.67
Completeness [%]	99.8	97.77
Redundancy	5.4	4.8
CC(1/2)	0.967	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, and 17% PEG3350