9IZN

Crystal structure of HKU1A RBD bound to TMPRSS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-11
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	63.324, 78.358, 257.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.400
R-factor	0.2167
Rwork	0.214
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.514
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.400	2.400
Number of reflections	51020	8031
<I/σ(I)>	12.74
Completeness [%]	99.7
Redundancy	9.93
CC(1/2)	0.998	0.295

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole (pH 7.0) and 8% w/v Polyethylene glycol 3350