9IXR
Crystal structure of OXA-10 variant A124T in the complex with ceftazidime
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.510, 99.040, 127.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.190 |
| R-factor | 0.16909 |
| Rwork | 0.167 |
| R-free | 0.20926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fof |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.769 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.144 | |
| Rmeas | 0.151 | |
| Rpim | 0.042 | 0.317 |
| Number of reflections | 32211 | 3169 |
| <I/σ(I)> | 15.63 | |
| Completeness [%] | 99.6 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 20% PEG 3350, 0.2 M Sodium formate, 0.1 M Tris-HCl pH 7.0 |
