9IGO
PR3 S203A I221N W222N G223T mutant in complex with the extracellular domain of CD177
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.760, 120.735, 61.983 |
| Unit cell angles | 90.00, 96.99, 90.00 |
Refinement procedure
| Resolution | 43.090 - 1.500 |
| R-factor | 0.1476 |
| Rwork | 0.146 |
| R-free | 0.18390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.143 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.370 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 92506 | 4607 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.7 |
| CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BIS-TRIS pH 6.5, 45% v/v Polypropylene glycol P 400 |
