9I6A
Crystal structure of human Cdc20 bound to synthetic D-box peptide D7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.003, 86.869, 48.027 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 45.240 - 1.915 |
| R-factor | 0.2078 |
| Rwork | 0.206 |
| R-free | 0.23070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.243 | 2.094 |
| High resolution limit [Å] | 1.915 | 1.915 |
| Rmerge | 0.132 | 1.161 |
| Rmeas | 0.147 | 1.299 |
| Rpim | 0.064 | 0.573 |
| Total number of observations | 76640 | 3657 |
| Number of reflections | 14979 | 750 |
| <I/σ(I)> | 7.6 | 1.5 |
| Completeness [%] | 71.8 | |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 10 % MPD, 12-14 % PEG 6000, 0.1 M MES pH 6.5 |
