9HXV

Crystal structure of TET2-DNA in complex with IOX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-25
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.873128
Spacegroup name	C 2 2 21
Unit cell lengths	48.440, 89.130, 268.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.560 - 1.900
R-factor	0.1912
Rwork	0.190
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Rmeas	0.106	1.141
Number of reflections	45640	6070
<I/σ(I)>	17.08	2.74
Completeness [%]	98.3	93.2
Redundancy	13.43	12.43
CC(1/2)	0.999	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	24 % PEG 2000MME, 100 mM MES pH 7.0 25 mg/mL protein 1:1 with DNA plus 5 mM IOX1 Seeding experiment (1 ul reservoir + 1 ul protein:DNA:IOX1 + 0.2 ul seeding solution)