9HJS

Crystal structure of human geranylgeranyl diphosphate synthase mutant R235C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.88560
Spacegroup name	P 1 2 1
Unit cell lengths	102.623, 69.081, 154.987
Unit cell angles	90.00, 97.39, 90.00

Refinement procedure

Resolution	45.190 - 2.510
R-factor	0.2284
Rwork	0.227
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.791
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.327	2.699
High resolution limit [Å]	2.510	2.510
Number of reflections	56879	2844
<I/σ(I)>	12.5
Completeness [%]	77.2
Redundancy	3.5
CC(1/2)	0.999	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1M sodium cacodylate pH 6.6, 40% MPD, 5% PEG 8000, 0.1M spermine