Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.030, 157.920, 181.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.050 - 2.310 |
| R-factor | 0.2121 |
| Rwork | 0.211 |
| R-free | 0.23520 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.437 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 2.330 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.114 | 3.032 |
| Rmeas | 0.123 | 3.269 |
| Rpim | 0.046 | 1.214 |
| Number of reflections | 256190 | 8440 |
| <I/σ(I)> | 9.92 | 0.47 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.1 | |
| CC(1/2) | 1.000 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 4% PEG 4K, 13% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |
