9GVU

Human Rab27A in complex with cyclic peptide IMP-2660

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	70.357, 72.714, 91.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.970 - 2.100
R-factor	0.189
Rwork	0.186
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.098
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.970	2.172
High resolution limit [Å]	2.097	2.097
Number of reflections	28187	2727
<I/σ(I)>	4.39
Completeness [%]	99.6
Redundancy	12.5
CC(1/2)	0.951	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.15 M NH4SO4, 0.1 MES, pH 6.0, 15% PEG 4K