9GSU

Structure of PP1-Neurabin bound to 4E-BP1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	104.955, 130.647, 156.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.480 - 2.360
R-factor	0.2436
Rwork	0.242
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.597
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5058)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.480	2.420
High resolution limit [Å]	2.350	2.350
Number of reflections	44376	3023
<I/σ(I)>	5.29
Completeness [%]	98.9
Redundancy	27.2
CC(1/2)	0.990	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 6000, 0.2M MgCl2, 0.1 M MES pH 6.0