9ESA

Aurora-C with SER mutation in complex with INCENP peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-04-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5405
Spacegroup name	C 2 2 21
Unit cell lengths	79.280, 79.490, 265.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.800
R-factor	0.2313
Rwork	0.228
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.279
Data reduction software	CrystalClear (1.3.6sp3)
Data scaling software	CrystalClear (1.3.6sp3)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.870	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.126	0.350
Number of reflections	21012	2057
<I/σ(I)>	6.1	2.3
Completeness [%]	99.6	99.9
Redundancy	4.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Bis-Tris pH5.5, 0.025-0.05 M ammonium sulphate, 9-12% PEG3350