9EMZ
13C/15N-labelled Integral Membrane Enzyme LspA in the Lipid Cubic Phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.991, 106.086, 85.919 |
| Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
| Resolution | 60.000 - 3.000 |
| R-factor | 0.2484 |
| Rwork | 0.245 |
| R-free | 0.30330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.605 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 85.176 | 85.176 | 3.307 |
| High resolution limit [Å] | 2.995 | 9.940 | 2.995 |
| Rmerge | 0.121 | 0.028 | 0.769 |
| Number of reflections | 10425 | 520 | 522 |
| <I/σ(I)> | 4.3 | 2.3 | 1.5 |
| Completeness [%] | 72.7 | 67.3 | |
| Redundancy | 2.3 | ||
| CC(1/2) | 0.990 | 1.000 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM MES pH 5.6-6.0, 35-43 %(v/v) PEG400 and 60-100 mM ammonium phosphate monobasic |
