9EK5
TRIM21 PrySpry domain bound to an enhanced PRLX-93936 analog
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.317, 99.317, 50.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.326 - 2.100 |
| Rwork | 0.195 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.507 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.320 | 1.930 |
| High resolution limit [Å] | 1.840 | 1.900 |
| Number of reflections | 22542 | 1015 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 26.1 | |
| CC(1/2) | 0.998 | 0.344 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 4.2 M Sodium Formate; 100 mM Tris-HCl, pH 7.5 |
