9ECK

Estrogen receptor alpha

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2
Spacegroup name	P 1
Unit cell lengths	50.615, 59.408, 100.850
Unit cell angles	94.05, 93.83, 115.36

Refinement procedure

Resolution	23.770 - 3.200
R-factor	0.2167
Rwork	0.212
R-free	0.29280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.986
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.400
High resolution limit [Å]	3.200	3.200
Number of reflections	15703	12000
<I/σ(I)>	2.3
Completeness [%]	90.6
Redundancy	3.5
CC(1/2)	0.800	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	PEG3350