9DOD
Crystal structure of PRMT5:MEP50 in complex with PRT543
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.567, 139.202, 178.548 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.950 - 2.500 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.251 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.950 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.600 |
| Rmeas | 0.062 | 0.622 |
| Rpim | 0.017 | 0.162 |
| Number of reflections | 44312 | 4599 |
| <I/σ(I)> | 25.4 | 2.3 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 14 | |
| CC(1/2) | 0.984 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M Na-Cit,0.2M NaAc,11% PEG4000,pH6.0 |
