9DAF

L-asparaginase II (EcA2-K12)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97718
Spacegroup name	P 1 21 1
Unit cell lengths	59.746, 123.051, 79.071
Unit cell angles	90.00, 108.98, 90.00

Refinement procedure

Resolution	47.509 - 2.350
Rwork	0.173
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.723
Data reduction software	autoPROC
Data scaling software	autoPROC
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.510	47.510
High resolution limit [Å]	2.350	2.350
Number of reflections	44971	3139
<I/σ(I)>	14.57
Completeness [%]	99.9
Redundancy	20
CC(1/2)	0.843	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	298	0.2 M Ammonium acetate, 20% w/v Polyethylene glycol 3,350.