9CRA

CryoEM Structure of the C-terminally truncated form of human NAD Kinase bound to NAD

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	TFS Selectris X
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.34
Number of Particle	1716164
Software	cryoSPARC,Coot,SerialEM,UCSF ChimeraX,PHENIX
Symmetry	D2
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION