9BP6

Structure of alpha1B and betaI/IVb microtubule bound to GMPCPP

Replaces:  8V2I

EM specimen

Vitrification	Yes
Instrument	LEICA EM GP

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.1
Number of Particle	124408
Software	RELION,ISOLDE,SerialEM,Gctf,Coot,PHENIX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION