9AYC
Tetra-phosphorylated, E1435Q Ycf1 mutant in inward-facing wide conformation
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.23 |
| Number of Particle | 4840688 |
| Software | cryoSPARC,ISOLDE,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
