9OP3

Structure of Human ADAR2-R2D complexed with dsRNA containing 8-azaN and 2'-Deoxy-2'-fluorouridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	170.000, 63.210, 142.200
Unit cell angles	90.00, 118.13, 90.00

Refinement procedure

Resolution	65.700 - 2.590
R-factor	0.1799
Rwork	0.178
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.158
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.000	2.720
High resolution limit [Å]	2.590	2.590
Rmerge	0.081	0.719
Number of reflections	31299	1565
<I/σ(I)>	7.8	1.1
Completeness [%]	93.5	70
Redundancy	3.6
CC(1/2)	0.997	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	Reservoir Buffer: 250 mM NaCl, 5 mM MOPS, 20% PEG 4K