9MK3
Engineered AsCas12a (M537R, F870L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.916, 198.585, 140.363 |
| Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
| Resolution | 49.276 - 2.698 |
| Rwork | 0.196 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.82)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.860 |
| High resolution limit [Å] | 2.698 | 2.698 |
| Number of reflections | 121265 | 18529 |
| <I/σ(I)> | 10.8 | 1.11 |
| Completeness [%] | 98.1 | |
| Redundancy | 5.75 | |
| CC(1/2) | 0.998 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na Acetate, pH 4.3, 6% polyethylene glycol (PEG) 3,350, 10% hexanediol |
