9M1Z

Crystal Structure of MAP2K6 complexed with 5Z-7-oxozeaenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	68.347, 120.155, 106.317
Unit cell angles	90.00, 101.01, 90.00

Refinement procedure

Resolution	47.860 - 3.000
R-factor	0.2771
Rwork	0.274
R-free	0.33160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.861	3.120
High resolution limit [Å]	2.950	2.950
Number of reflections	34340	5348
<I/σ(I)>	4.83
Completeness [%]	95.7
Redundancy	3
CC(1/2)	0.992	0.251

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Citrate pH5.0, 20% PEG4000, 0.2 M L-proline