9L89
Structure of human TREX1-DNA complex with K160R disease-associated mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-10-22 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.777, 91.293, 158.241 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 43.770 - 2.380 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tqq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.613 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.770 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rpim | 0.150 | 0.767 |
| Number of reflections | 49774 | 4725 |
| <I/σ(I)> | 4.3 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.927 | 0.413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M Li2SO4, 27.5% PEG 3350, 0.1 M Bis-Tris pH 6.5 |
