9BJU
Crystal structure of the complex between VHL, ElonginB, ElonginC, and compound 5
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2018-05-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97893 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 93.297, 93.297, 362.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.860 - 2.470 |
R-factor | 0.2203 |
Rwork | 0.218 |
R-free | 0.25565 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.374 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.560 |
High resolution limit [Å] | 2.470 | 5.320 | 2.470 |
Rmerge | 0.136 | 0.074 | 1.228 |
Rmeas | 0.142 | 0.078 | 1.281 |
Rpim | 0.040 | 0.023 | 0.358 |
Number of reflections | 58657 | 6328 | 5725 |
<I/σ(I)> | 4.5 | ||
Completeness [%] | 99.9 | 99.6 | 100 |
Redundancy | 13.1 | 12.3 | 12.5 |
CC(1/2) | 0.989 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 mM sodium cacodylate pH 5.7, 15% PEG8000, 0.2 M magnesium acetate, 5 mM DTT |