9B12
Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.96863 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.413, 70.191, 193.944 |
Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
Resolution | 46.150 - 1.810 |
R-factor | 0.2331 |
Rwork | 0.231 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.660 | 2.140 |
High resolution limit [Å] | 1.810 | 2.100 |
Rmerge | 0.074 | 0.697 |
Rmeas | 0.089 | 0.840 |
Rpim | 0.050 | 0.464 |
Total number of observations | 13825 | |
Number of reflections | 84203 | 4419 |
<I/σ(I)> | 6.6 | 1.1 |
Completeness [%] | 99.7 | |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.995 | 0.682 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 3:1 with reservoir solution containing 50% PEG 200 and 0.1 M HEPES pH 7.5 |