8ZR1

Cocrystallization of engineered streptavidin with A9 oligo DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-08
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	46.084, 58.006, 57.791
Unit cell angles	90.65, 107.54, 98.84

Refinement procedure

Resolution	37.662 - 2.601
R-factor	0.2016
Rwork	0.198
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lng
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.198	0.087	0.514
Rmeas		0.123	0.727
Rpim	0.140	0.087	0.514
Number of reflections	16710	1672	1670
<I/σ(I)>	6
Completeness [%]	97.5	97.5	97.3
Redundancy	1.9	1.9	1.9
CC(1/2)		0.974	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	298	10 mM Tris-HCl