8ZR1

Cocrystallization of engineered streptavidin with A9 oligo DNA

Summary for 8ZR1

Entry DOI	10.2210/pdb8zr1/pdb
Descriptor	Streptavidin (2 entities in total)
Functional Keywords	biotin-binding protein, unknown function
Biological source	Streptomyces avidinii
Total number of polymer chains	4
Total formula weight	56749.92
Authors	Minamihata, K.,Adachi, M. (deposition date: 2024-06-03, release date: 2025-06-04)
Primary citation	Nagatani, A.,Minamihata, K.,Adachi, M.,Wakabayashi, R.,Goto, M.,Kamiya, N. Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags. Rsc Adv, 15:6817-6822, 2025 Cited by PubMed Abstract: We have achieved a novel co-crystal in which the dual functions of the protein and single-stranded DNA are maintained by introducing a charged peptide tag at the C-terminus of the protein. The functionalities allowed the co-crystals to be modified with high selectivity. Additionally, we have confirmed that energy transfer occurs between the two molecules modified within the co-crystal. Therefore, this co-crystal has the potential as a novel biomaterial applicable to biosensors. PubMed: 40035016 DOI: 10.1039/d4ra08326a PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.601 Å)

Structure validation