6LNG

Rapid crystallization of streptavidin using charged peptides

Summary for 6LNG

Entry DOI	10.2210/pdb6lng/pdb
Descriptor	Streptavidin, GLYCEROL (3 entities in total)
Functional Keywords	biotin-binding protein, peptide binding protein
Biological source	Streptomyces avidinii
Total number of polymer chains	6
Total formula weight	79039.43
Authors	Minamihata, K.,Tsukamoto, K.,Adachi, M.,Shimizu, R.,Mishina, M.,Kuroki, R.,Nagamune, T. (deposition date: 2019-12-30, release date: 2020-03-18, Last modification date: 2023-11-22)
Primary citation	Minamihata, K.,Tsukamoto, K.,Adachi, M.,Shimizu, R.,Mishina, M.,Kuroki, R.,Nagamune, T. Genetically fused charged peptides induce rapid crystallization of proteins. Chem.Commun.(Camb.), 56:3891-3894, 2020 Cited by PubMed Abstract: We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials. PubMed: 32134050 DOI: 10.1039/c9cc09529b PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.80000150972 Å)

Structure validation