8ZCA

Crystal structure of human CD47 ECD bound to Fab of Hu1C8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	45.224, 79.931, 92.751
Unit cell angles	112.50, 96.61, 103.16

Refinement procedure

Resolution	38.520 - 2.500
R-factor	0.2174
Rwork	0.215
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.163	0.087	0.357
Rmeas	0.197	0.105	0.449
Rpim	0.109	0.058	0.268
Total number of observations	108179
Number of reflections	38158	1917	1671
<I/σ(I)>	4.8
Completeness [%]	97.7	97.6	86.9
Redundancy	2.8	3.1	2.2
CC(1/2)	0.926	0.980	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M HEPES (pH 7.0) and 20% (w/v) polyethylene glycol 3350