8YUC
Crystal structure of SARS-CoV-2 ConSp RBD in complex with antibodies PDI222 Fab and COVA1-16 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 127.264, 127.264, 240.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.520 - 4.510 |
| R-factor | 0.2764 |
| Rwork | 0.275 |
| R-free | 0.30780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7rr0 7lm8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.600 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 13873 | 901 |
| <I/σ(I)> | 5.73 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 20.3 | |
| CC(1/2) | 0.993 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0 M NH4-sulfate |
