7RR0
SARS-CoV-2 receptor binding domain bound to Fab PDI 222
Summary for 7RR0
| Entry DOI | 10.2210/pdb7rr0/pdb |
| EMDB information | 24649 |
| Descriptor | Spike protein S1, PDI 222 Fab Heavy Chain, PDI 222 Fab Light Chain, ... (4 entities in total) |
| Functional Keywords | antibody, sars-cov-2 rbd, complex, viral protein, viral protein-immune system complex, viral protein/immune system |
| Biological source | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) More |
| Total number of polymer chains | 3 |
| Total formula weight | 47124.62 |
| Authors | Pymm, P.,Glukhova, A.,Black, K.A.,Tham, W.H. (deposition date: 2021-08-08, release date: 2021-10-06, Last modification date: 2024-11-13) |
| Primary citation | Wheatley, A.K.,Pymm, P.,Esterbauer, R.,Dietrich, M.H.,Lee, W.S.,Drew, D.,Kelly, H.G.,Chan, L.J.,Mordant, F.L.,Black, K.A.,Adair, A.,Tan, H.X.,Juno, J.A.,Wragg, K.M.,Amarasena, T.,Lopez, E.,Selva, K.J.,Haycroft, E.R.,Cooney, J.P.,Venugopal, H.,Tan, L.L.,O Neill, M.T.,Allison, C.C.,Cromer, D.,Davenport, M.P.,Bowen, R.A.,Chung, A.W.,Pellegrini, M.,Liddament, M.T.,Glukhova, A.,Subbarao, K.,Kent, S.J.,Tham, W.H. Landscape of human antibody recognition of the SARS-CoV-2 receptor binding domain. Cell Rep, 37:109822-109822, 2021 Cited by PubMed Abstract: Potent neutralizing monoclonal antibodies are one of the few agents currently available to treat COVID-19. SARS-CoV-2 variants of concern (VOCs) that carry multiple mutations in the viral spike protein can exhibit neutralization resistance, potentially affecting the effectiveness of some antibody-based therapeutics. Here, the generation of a diverse panel of 91 human, neutralizing monoclonal antibodies provides an in-depth structural and phenotypic definition of receptor binding domain (RBD) antigenic sites on the viral spike. These RBD antibodies ameliorate SARS-CoV-2 infection in mice and hamster models in a dose-dependent manner and in proportion to in vitro, neutralizing potency. Assessing the effect of mutations in the spike protein on antibody recognition and neutralization highlights both potent single antibodies and stereotypic classes of antibodies that are unaffected by currently circulating VOCs, such as B.1.351 and P.1. These neutralizing monoclonal antibodies and others that bind analogous epitopes represent potentially useful future anti-SARS-CoV-2 therapeutics. PubMed: 34610292DOI: 10.1016/j.celrep.2021.109822 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.12 Å) |
Structure validation
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