8YQ0

Crystal structure of human phosphoribosyl pyrophosphate synthetase 1(PRPS1) chimera swapped with three residues from PRPS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9873
Spacegroup name	P 43
Unit cell lengths	108.083, 108.083, 659.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 3.100
R-factor	0.199
Rwork	0.196
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h06
RMSD bond length	0.012
RMSD bond angle	1.711
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.538
Rmeas	0.559
Rpim	0.153
Number of reflections	135705	13496
<I/σ(I)>	2.2
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)		0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Lithium sulfate, 20% PEG 3350