8YPY

Crystal strcuture of human phosphoribosyl pyrophosphate synthetase2 (PRPS2) in complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9873
Spacegroup name	P 1 21 1
Unit cell lengths	94.701, 73.598, 170.500
Unit cell angles	90.00, 94.44, 90.00

Refinement procedure

Resolution	46.910 - 2.740
R-factor	0.273
Rwork	0.272
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h06
RMSD bond length	0.025
RMSD bond angle	2.345
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.910	2.800
High resolution limit [Å]	2.740	2.750
Number of reflections	54437	2202
<I/σ(I)>	9.6
Completeness [%]	94.1
Redundancy	4.2
CC(1/2)	0.689	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Lithium sulfate, 35% MPD