8YLW

Crystal Structure of Lysyl-tRNA Synthetase from Plasmodium falciparum complexed with Lys-AMS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 2
Unit cell lengths	59.360, 302.190, 141.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.526 - 2.500
R-factor	0.212
Rwork	0.209
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pg3
RMSD bond length	0.009
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc1_3423-0000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	2.650
High resolution limit [Å]	2.500	2.500
Rmeas	0.126	2.170
Number of reflections	89576	14235
<I/σ(I)>	14.51	0.93
Completeness [%]	100.0	99.9
Redundancy	13.7
CC(1/2)	1.000	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Bis-Tris, pH 6.5, 2% v/v Tascimate, pH 6.0 and 20% w/v PEG 3350