8YJF
Structure of human SPT16 MD-CTD and MCM2 HBD chaperoning a histone H3-H4 tetramer and an H2A-H2B dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 61 |
Unit cell lengths | 190.405, 190.405, 79.832 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.130 - 4.400 |
R-factor | 0.3571 |
Rwork | 0.293 |
R-free | 0.31220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.642 |
Data reduction software | autoPX (v1.0) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.130 | 4.920 |
High resolution limit [Å] | 4.400 | 4.400 |
Number of reflections | 10698 | 3002 |
<I/σ(I)> | 11.7 | |
Completeness [%] | 99.9 | |
Redundancy | 11.2 | |
CC(1/2) | 0.998 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M calcium acetate, 0.1 M Sodium cacodylate pH 5.5, 12% (w/v) PEG 8000 |